Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria
نویسندگان
چکیده
منابع مشابه
Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria
Hemolytic assay, bacterial adhesion to hydrocarbons (BATH) assay, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing bacteria. 105 bacterial strains were screened for their biosurfactant production. Results from screening methods revealed that hemolytic and BATH assa...
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Marine microorganisms have unique physiological properties and novel metabolites such as biosurfactant to live in extreme habitats (e.g. oil polluted environment). The aim of this study was to characterize some biosurfactant-producing bacteria collected from sediment and seawater samples from the Caspian Sea. These bacteria were isolated using enrichment method in ONR7a medium with crude oil as...
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Different screening methods namely, hemolytic assay (HA), modified drop collapse (MDC), tilted glass slide (TGST), oil spread method (OSM), blue agar plate (BAP), hydrocarbon overlaid agar (HOA) plate, emulsification index (EI), emulsification assay (EA) were assessed for their efficiency to detect biosurfactant producing marine bacteria. Forty-five strains of bacteria, comprising 18 Acinetobac...
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This chapter gives an overview of current methods for the isolation of biosurfactant producing microbes. The common screening methods for biosurfactants are presented. Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Hydrocarbon-contaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains hav...
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ژورنال
عنوان ژورنال: Journal of Petroleum & Environmental Biotechnology
سال: 2013
ISSN: 2157-7463
DOI: 10.4172/2157-7463.s1-001